Abstract
This study describes a bacterium strain RBPA9 isolated from a municipality waste dumping area capable of degrading phenol, proposed as a novel species ofPseudomonas. Cells are Gram-negative, rod shaped, aerobic and motile. The genome is 3.92Mb, and the G + C content is 64.64%. The overall genome relatedness indices such as in silico DNA-DNA hybridization (isDDH), average nucleotide identity (ANI), and average amino acid identity (AAI) values were below 70% and 95-96%, respectively. Phylogenetic analysis based on genome-wide core genes and 16S rRNA gene sequences revealed that strain RBPA9 clustered withPseudomonas stutzeriATCC 17588T in both the phylogenetic trees. Maximum growth was recorded at 200mg /L concentration of phenol which was consumed within 24h. A gene cluster of phenol degradation pathway was detected. The quantitative real-time PCR (RT-PCR) demonstrated the expression of all the genes required in the meta-cleavage pathway of phenol in RBPA9. Our results reveal that strain RBPA9 represents a novel species for whichPseudomonas phenolilyticasp. nov. is proposed. The type strain is RBPA9T (= TBRC 15231T = NBRC 115284T).
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