Pseudomonas nanhaiensis sp. nov., a lipase-producing bacterium isolated from deep-sea sediment of the South China Sea.

Yu Pang,Min Lin,Wei Zhang,Yage Zhang,Zhengfu Zhou,Wei Lu,Mengru Chen,Yongliang Yan,Ming Chen

doi:10.1007/s10482-021-01639-y

Abstract

A bacterial lipase producing bacterium, designated SCS 2-3, was isolated from deep-sea sediment of the South China Sea. Phylogenetic analysis based on the 16S rRNA sequence revealed that strain SCS2-3 belonged to the genus Pseudomonas and had 98.56% similarity to P. xinjiangensis NRRL B-51270T as the closest relative strain. MLSA using four protein-coding genes (dnaK, gyrA, recA, and rpoB) showed strain SCS 2-3 to form a separate branch. ANI and in silico DDH values between strain SCS 2-3 and related type strains of Pseudomonas were less than 81.51% and 23.80%, respectively. Genome comparison showed that strain SCS 2-3 shared 1875 core gene families with other eight closely related type strains in Pseudomonas, and the number of strain-unique genes was 263. Through gene annotations, genes related to lipase were found in the genome. Furthermore, a combination of phenotypic, chemotaxonomic, phylogenetic and genotypic data clearly indicated that strain SCS 2-3 represents a novel species of the genus Pseudomonas, for which the name Pseudomonas nanhaiensis sp. nov. is proposed. The type strain is SCS 2-3T (= GDMCC 1.2219T = JCM 34440T).

Highlights

Pseudomonas is one of the most studied species of bacteria and is the genus with the largest number of species(George et al, 2005)
Strain SCS 2–3 was isolated from deep sea sediment sample from the South China Sea
The strain SCS 2–3 was capable of growth at temperatures between 10°C and 45°C, and the strain grew well at pH values between 5.0 and 10.0

Summary

Introduction

Pseudomonas is one of the most studied species of bacteria and is the genus with the largest number of species(George et al, 2005). The name Pseudomonas was first created by professor Migula of the Karlsruhe Institute in Germany at the end of 19th century(Migula, 1895). They are Gram-negative, rod-shaped and polar-flagellated bacteria. At the time of writing, genus Pseudomonas includes about 396 species with 21 subspecies Pseudomonas species are ubiquitous environmental organisms that occupy several niches, including soils, marine environments, fresh water, plants and animals(Khan et al, 2009; Peix et al, 2009). Pseudomonas species are of great interest for biotechnological applications due to the versatile metabolic machinery and their potential for adaptation to fluctuating environmental conditions(Novik et al, 2015). Some species can promote plant-growth(Ke et al, 2019), whereas others act as pathogens to plants(Oueslati et al, 2019) and animals(Driscoll et al, 2007)

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