Abstract
Initially described in 1990, Pseudomonas fluorescens HK44 served as the first whole-cell bioreporter genetically endowed with a bioluminescent (luxCDABE) phenotype directly linked to a catabolic (naphthalene degradative) pathway. HK44 was the first genetically engineered microorganism to be released in the field to monitor bioremediation potential. Subsequent to that release, strain HK44 had been introduced into other solids (soils, sands), liquid (water, wastewater), and volatile environments. In these matrices, it has functioned as one of the best characterized chemically-responsive environmental bioreporters and as a model organism for understanding bacterial colonization and transport, cell immobilization strategies, and the kinetics of cellular bioluminescent emission. This review summarizes the characteristics of P. fluorescens HK44 and the extensive range of its applications with special focus on the monitoring of bioremediation processes and biosensing of environmental pollution.
Highlights
Bioreporters are genetically engineered cells that harbor genetic fusions between responsive gene promoters and reporter genes
This review summarizes the broad range of studies carried out with the bioluminescent bioreporter
The indigenous nature of strain HK44 in combination with target inducible bioluminescence has enabled its service in a series of bio-analytical applications for the detection of polycyclic aromatic hydrocarbons (PAHs) and other chemical targets in soil, water, and air
Summary
Bioreporters are genetically engineered cells (predominantly microbial) that harbor genetic fusions between responsive gene promoters and reporter genes. Bioreporters enable monitoring of metabolic gene expression or repression via measurement of the signal resulting from the joint expression of the promoter and reporter genes. Catabolic (inductive) bioreporters express their reporter genes upon external activation by chemical, physical or biological inducers, and increase their light emission intensity in relation to the concentration of inducer present. Catabolic bioreporters have a wide range of applications in sensing and monitoring strategies, and can be designed to either nonspecifically or report on inducer interactions. The bioreporter responds via an increase in signal linked to general stress or DNA damage effects related to exposure to a cytotoxic, genotoxic, or mutagenic chemical or physical interaction. The extensive and divergent use of this model living bacterial bioreporter will be examined in this review to provide a critical overview of the advantages and disadvantages of bioreporter sensing and monitoring technologies
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
