Abstract
The bacterial strain 2-92T, isolated from a field plot under long-term (>40 years) mineral fertilization, exhibited in vitro antagonistic properties against fungal pathogens. A polyphasic approach was undertaken to verify its taxonomic status. Strain 2-92T was Gram-reaction-negative, aerobic, non-spore-forming, motile by one or more flagella, and oxidase-, catalase- and urease-positive. The optimal growth temperature of strain 2-92T was 30 °C. 16S rRNA gene sequence analysis demonstrated that the strain is related to species of the genus Pseudomonas. Phylogenetic analysis of six housekeeping genes (dnaA, gyrB, recA, recF, rpoB and rpoD) revealed that strain 2-92T clustered as a distinct and well separated lineage with Pseudomonassimiae as the most closely related species. Polar lipid and fatty acid compositions corroborated the taxonomic position of strain 2-92T in the genus Pseudomonas. Phenotypic characteristics from carbon utilization tests could be used to differentiate strain 2-92T from closely related species of the genus Pseudomonas. DNA–DNA hybridization values (wet laboratory and genome-based) and average nucleotide identity data confirmed that this strain represents a novel species. On the basis of phenotypic and genotypic characteristics, it is concluded that this strain represents a separate novel species for which the name Pseudomonas canadensis sp. nov. is proposed, with type strain 2-92T (=LMG 28499T=DOAB 798T). The DNA G+C content is 60.30 mol%.
Highlights
The bacterial strain 2-92T, isolated from a field plot under long-term (>40 years) mineral fertilization, exhibited in vitro antagonistic properties against fungal pathogens
All 12 biological control isolates were identified as members of the genus Pseudomonas on the basis of phenotypic features and 16S rRNA sequence analyses
The GenBank accession numbers of draft genomes used in this study and from which the sequences of six genes were extracted are AYTD00000000, MDDQ00000000, MDDR00000000, MDGK00000000, MDFK00000000, MDFI00000000, MDFH00000000 and MDFJ00000000 for strain 2-92T, Pseudomonas azotoformans LMG 21611T, P. costantinii LMG 22119T, P. extremorientalis LMG 19695T, P. poae LMG 21465T, P. salomonii LMG 22120T, P. simiae CCUG 50988T and P. trivialis LMG 21464T, respectively
Summary
The bacterial strain 2-92T, isolated from a field plot under long-term (>40 years) mineral fertilization, exhibited in vitro antagonistic properties against fungal pathogens. The GenBank accession numbers of draft genomes used in this study and from which the sequences of six genes (dnaA, gyrB, recA, recF, rpoB and rpoD) were extracted are AYTD00000000, MDDQ00000000, MDDR00000000, MDGK00000000, MDFK00000000, MDFI00000000, MDFH00000000 and MDFJ00000000 for strain 2-92T, Pseudomonas azotoformans LMG 21611T, P. costantinii LMG 22119T, P. extremorientalis LMG 19695T, P. poae LMG 21465T, P. salomonii LMG 22120T, P. simiae CCUG 50988T and P. trivialis LMG 21464T, respectively.
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