Pseudomonas bacteriophage AN14 a Baikal-borne representative of Yuavirus

Abstract

Siphoviruses with a prolate capsid infecting Pseudomonas sp. were isolated from environmental sources through the world, and were recently defined as a separate taxonomic genus Yuavirus. Besides a distinguishable morphology, the hallmarks of the genus are heavily modified genomic DNA and a lytic infection cycle while putative lysogeny genes are proposed in the genomes. Bacteriophage AN14 was isolated from Lake Baikal ecosystem and was assigned as a member of Yuavirus. We report the biological and morphological features if this phage, as well as the comprehensive reannotation of its genome. Modern bioinformatics and phylogenomic analysis resulted in the refinement of the taxonomic attribution of the YuA-like phages and highlighting the specific genomic and proteomic features typical for Yuavirus phages including AN14.