Abstract
Strain RA15T was isolated from the rhizosphere of the halophyte plant Arthrocnemum macrostachyum growing in the Odiel marshes (Huelva, Spain). RA15T cells were Gram stain-negative, non-spore-forming, aerobic rods and formed cream-coloured, opaque, mucoid, viscous, convex, irregular colonies with an undulate margin. Optimal growth conditions were observed on tryptic soy agar (TSA) plates supplemented with 2.5 % NaCl (w/v) at pH 7.0 and 28 °C, although it was able to grow at 4–32 °C and at pH values of 5.0–9.0. The NaCl tolerance range was from 0 to 15 %. The major respiratory quinone was Q8 but Q9 was also present. The most abundant fatty acids were summed feature 3 (C16 : 1 ω7c and/or C16 : 1 ω6c), C17 : 1 ω8c and C16 : 0. The polar lipids profile comprised phosphatidylglycerol and phosphatidylethanolamine as the most abundant representatives. Phylogenetic analyses confirmed the well-supported affiliation of strain RA15T within the genus Pseudoalteromonas , close to the type strains of Pseudoalteromonas neustonica , Pseudoalteromonas prydzensis and Pseudoalteromonas mariniglutinosa . Results of comparative phylogenetic and phenotypic studies between strain RA15T and its closest related species suggest that RA15T could be a new representative of the genus Pseudoalteromonas , for which the name Pseudoalteromonas rhizosphaerae sp. nov. is proposed. The type strain is RA15T (=CECT 9079T=LMG 29860T). The whole genome has 5.3 Mb and the G+C content is 40.4 mol%.
Highlights
Pseudoalteromonas [1] is the type genus of the family Pseudoalteromonadaceae [2]
Phylogenetic analyses confirmed the well-supported affiliation of strain RA15T within the genus Pseudoalteromonas, close to the type strains of P. neustonica, P. prydzensis, and P. mariniglutinosa
Strain RA15T was isolated from the rhizosphere of Arthrocnemum macrostachyum plants growing in the Odiel marshes (Huelva, Spain) [15]. 16S rRNA gene sequence analysis showed that it clustered within the genus Pseudoalteromonas with a 97.6% of similarity to the type strain of Pseudoalteromonas prydzensis [15]
Summary
Pseudoalteromonas [1] is the type genus of the family Pseudoalteromonadaceae [2]. It is currently shaped by 47 species [3] characterized by presenting Gram staining-negative, motile, non-spore forming, aerobic, oxidase positive rods [4] requiring Na+ ion for growth [5] whose type species is Pseudoalteromonas haloplanktis [1,4,6]. Optimal growth conditions were observed on TSA plates supplemented with 2.5% NaCl (w/v) at pH 7.0 and 28oC, it was able to grow between 4oC-32oC and pH values of 5.0-9.0.
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More From: International Journal of Systematic and Evolutionary Microbiology
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