Pseudaphelenchus chunanensis n. sp. (Tylenchina: Aphelenchoididae) found in Pinus massoniana from China

Abstract

Summary Pseudaphelenchus chunanensis n. sp. was isolated in branches of Pinus massoniana from eastern China. The new species is characterised by very short males (402 (360-452) μm) and females (470 (397-540) μm), three incisures in the lateral field, slender stylet with small but conspicuous basal knobs, excretory pore located at the level of anterior margin of the metacorpus or more anterior. Male spicules paired, thorn-like, separate, condylus truncate, sometimes broadly rounded, rostrum conical or triangular with bluntly pointed tip, calomus smoothly tapering together with lamina, thin and strongly arcuate in middle then tapering towards bluntly pointed distal tip, cucullus absent. A long and narrow bursa surrounds the entire tail but inconspicuous in most males. Conical tail bluntly rounded but sometimes has a short mucron. Female tail conical, smoothly tapering and slightly ventrally bent in distal part of tail, terminus fine or bluntly pointed. It is typologically different from six known Pseudaphelenchus species, and detailed phylogenetic analysis based on partial 18S ribosomal DNA (rDNA), internal transcribed spacer (ITS) and the D2-D3 region of 28S rDNA sequences further confirm this nematode as a new species.