PSCA gene expression in bladder, colorectal and prostate cancer patients from Basrah governorate southern of Iraq

Abstract

Cancer causes death in all countries of the world, with approximately (9.6) million deaths in 2018. Developing countries have high cancer rates. Furthermore, there are risk factors that lead to cancer, including economic and political instability and bad lifestyles. This is the first study of PSCA gene expression with BC, CRC and PC in Iraq, especially in Basrah governorate. This paper involved one hundred and one blood samples being collected from bladder, colorectal and prostate cancer. On the other hand, one hundred and one blood samples with no cancer were collected as a control group. Two ml of peripheral blood was drawn for RNA extraction, then total RNA was reverse transcribed to cDNA, and PSCA mRNA was measured using qPCR. The results showed PSCA gene was over expressed in bladder cancer (BC) with an expression level at ± 8.63 for patients and controls estimated with ±4.16. The gene expression was a 2-fold change in patients compared to healthy control. While the PSCA gene was over-expressed in colorectal cancer (CRC) with an expression level ± 8.16 for patients and controls estimated at ±3.30. That means the gene expression was a 2.47-fold change in patients compared to healthy control. The PSCA gene was over expressed in prostate cancer (PC) with an expression level ± 9.47 for patients and controls estimated at ±4.22. The gene expression was a 2.24-fold change in patients compared to healthy control. In the present study, PSCA gene was expressed at a significantly higher level in the BC, CRC and PC patients compared in the controls group.