PS-B07-2: EXERCISE TRAINING PREVENTS HIGH FRUCTOSE-INDUCED HYPERTENSION AND RENAL DAMAGES IN DAHL SALT-SENSITIVE RATS

Abstract

Background: High fructose diet (HFr) causes metabolic syndrome, and HFr-induced hypertension and renal damage are exaggerated in Dahl salt-sensitive (DS) rats. Exercise training (Ex) have antihypertensive and renal protective effects in rats fed HFr, but these effects of Ex are weak in DS rats fed a high-salt diet. This study thus examined the effects of Ex on DS rats fed HFr. Methods: Male DS rats were divided into three groups. The control group was fed a control diet, both the HFr group and the HFr-Ex group were fed a HFr (60% fructose). The HFr-Ex group also underwent treadmill running (20 m/min, 60 min/day, 5 day/ week). After 12 weeks, renal function, histology, and renin-angiotensin system (RAS) were examined. Results: HFr increased blood pressure, urinary albumin, and creatinine clearance, and Ex inhibited these increases. HFr induced glomerular sclerosis, podocyte injury, afferent arteriole thickening, and renal interstitial fibrosis, and Ex ameliorated them. HFr reduced plasma renin activity, and Ex further reduced the activity. HFr also increased the expression of angiotensinogen, renin, angiotensin converting enzyme (ACE), and angiotensin II type1 receptor (AT1R), and Ex restored the ACE expression to the control levels. HFr decreased the expression of ACE2, angiotensin II type2 receptor (AT2R), and Mas receptor (MasR), and Ex restored the ACE2 and MasR expressions to the control levels and further decreased the AT2R expression. HFr increased the ACE activity and decreased the ACE2 activity, and Ex restored these activities to the control levels. Conclusion: Ex prevents HFr-induced hypertension and renal damages in DS rats. The changes in renal RAS may be involved in the mechanism of the antihypertensive and renal protective effects of Ex.