Abstract
Purpose: To identify the biological function of phosphoserine aminotransferase 1 (PSAT1) in regulating cell proliferation and apoptosis in multiple myeloma (MM).Methods: The mRNA and protein levels of PSAT1 were determined using quantitative real-time polymerase chain reaction (PCR) and western blotting, respectively. Cell proliferation was measured using CCK-8 assay.Results: PSAT1 mRNA and protein expression levels were significantly increased in MM cell lines when compared to control cells. Moreover, downregulation of PSAT1 inhibited MM cell proliferation and induced cell apoptosis, whereas overexpression of PSAT1 promoted MM cell proliferation and suppressed cell apoptosis. Further analysis demonstrated that the underlying mechanism was via regulation of PI3K/AKT pathway.Conclusion: The results identified a novel role for PSAT1 in the progression of MM, which may provide a therapeutic and a new anticancer target for the therapy of MM.
 Keywords: Multiple myeloma, PSAT1, Cell proliferation, PI3K/AKT pathway
Highlights
Multiple myeloma (MM) is considered the second most common hematological malignancy worldwide whose clinical manifestations include hypercalcemia, renal insufficiency, anemia, infection and uncontrolled proliferation of monoclonal plasma cells [1]
phosphoserine aminotransferase 1 (PSAT1) was significantly overexpressed in MM cells
CCK-8 assay demonstrated that downregulation of PSAT1inhibited the proliferation of LP-1 cells that transfected with shPSAT1 when compared with that transfected with shNC (Figure 2 C)
Summary
Multiple myeloma (MM) is considered the second most common hematological malignancy worldwide whose clinical manifestations include hypercalcemia, renal insufficiency, anemia, infection and uncontrolled proliferation of monoclonal plasma cells [1]. PSAT1 has been shown to play a role in tumor cell proliferation, migration and chemo resistance, contributing to tumor progression and poor outcomes [12]. The mRNA and protein expression levels of PSAT1 in MM cell lines were measured and its effects on MM cell proliferation were analyzed. The results identified a novel role of PSAT1 in the progression of MM, which may provide a therapeutic and a new anticancer target for the therapy of MM. Multiple myeloma (MM) cell lines LP-1, NCI-H929, and U266 were obtained from Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Total protein was extracted in lysis buffer (Beyotime, China),separated by SDS-polyacrylamide gel electrophoresis, and blotted onto PVDF membranes [13].
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