PS-035 Aerobic Training But No Resistance Training Increases The Sirt3 Content And Reduces Adiposity In Sedentary Obese Male Adolescents

Abstract

Introduction Obesity is a risk factor for mitochondrial dysfunction and cardiovascular and metabolic diseases. In a previous research, we demonstrated that 12 weeks of aerobic training (AT) increased the content of SIRT3 protein in skeletal muscle of sedentary obese adolescents. SIRT3 improves mitochondrial function. There are not studies that have compared the effect of AT and resistance training (RT) on SIRT3 content. Object To compare the effect of AT versus RT on the content of SIRT3 protein in the muscle of sedentary obese adolescents. Methods Twenty-seven sedentary obese male adolescents (age: 16.7 ± 0.9 years; BMI: 33.7 ± 4.3 kg/m 2 ) completed a 1-month control period prior to be randomised to one of two supervised exercise protocols: AT (3 days/week, 40 min/day, 70–80% peak heart rate (HR peak ) or RT (11 exercises, 2 sets/exercise, 12 repetitions/set) for 12 weeks. Biopsies were obtained from the vastus lateralis muscle before and after 12 weeks of training protocol to analyse the content of SIRT3, PGC-1α and NRF-1 proteins by western blot. Participants were tested for VO 2peak , metabolic, and anthropometric variables. Results AT increased the content of SIRT3, which was associated with improvements in PGC-1α content (r = 0.7, p 2peak . RT increased arm and thigh muscle area and strength but did not affect SIRT3, PGC-1, and adiposity. Conclusions Our data suggest that AT was effective for improving the SIRT3 content and adiposity; therefore, AT may decrease the risk of cardiovascular and metabolic diseases.