PS-B07-5: EXERCISE TRAINING UPREGULATES FATTY ACID B;-OXIDATION WITH INCREASING PGC-1A AND PPARA IN THE KIDNEY OF DAHL SALT-SENSITIVE RATS

Abstract

Objective: Exercise training (Ex) has anti-hypertensive and renal protective effects. Fatty acid metabolism in the kidney is involved in renal damage. In this study, we investigated the effects of the Ex on blood pressure, renal damages, and fatty acid metabolism in Dahl salt-sensitive rats. Design and method: Six-week-old male Dahl salt-sensitive rats were divided into three groups: 1) normal salt diet (NS); 2) high salt diet (HS); 3) HS+Ex. NS or HS groups were fed diet containing 0.6% or 8% NaCl. Treadmill running was performed in HS+Ex group for 8 weeks (5 days/week; 60 min/day at 16–20 m/min, 0% grade). Systolic blood pressure (SBP) was monitored by tail-cuff method. Renal function, histology and the expression of enzymes and regulators for fatty acid metabolism were then assessed. Results: HS significantly elevated SBP, and Ex did not change SBP. HS significantly decreased creatinine clearance, and Ex significantly ameliorated creatinine clearance. HS significantly increased urinary protein excretion, and Ex significantly suppressed urinary protein excretion. HS induced glomerular sclerosis, and Ex suppressed them. HS decreased the renal expression of enzymes for fatty acid β;-oxidation including carnitine palmitoyltransferase type I (CPTI) and very long, medium and short-chain acyl-CoA dehydrogenases (CADs) and their transcription factors; peroxisome proliferator-activated receptor α (PPARα) and PPARγ-coactivator-1α (PGC-1α). Ex increased the renal expression of CPTI, CADs, PPARα and PGC-1α. Conclusions: Ex improves HS-induced renal damage with fatty acid β;-oxidation with increasing PPARα and PGC-1α in Dahl salt-sensitive rats in a systolic blood pressure-independent manner. These results suggest that Ex may have beneficial effects in HS-induced renal damage.