Abstract
In this research, microfluidic paper distance-based systems for the quantification of redox species are proposed. For the preparation of the sensing zone a Prussian Blue (PB) (convertible to Prussian White (PW)) layer was deposited in the channel manufactured by wax-printing technique. According to the chemical properties of PB/PW system, it is possible to develop optical sensors sensitive to both oxidizing and reducing agents. The created systems were evaluated for the determination of ascorbic acid and hydrogen peroxide, which were chosen and used as model analytes. The final versions of the proposed systems exhibited a linear response from 0.25 mmol L−1 to 4.0 and 2.0 mmol L−1 for ascorbic acid and H2O2, respectively. The analytical utility of the paper systems was confirmed by measuring the levels of ascorbic acid in dietary supplements. Results correlation obtained for the described systems and the reference method was over 0.98 (Pearson’s R-coefficient). All measurements were characterized by satisfactory reproducibility and acceptable uncertainty (RSD (%) < 6%). Finally, it was demonstrated that the modification of the PW-strip systems with oxidoreductase led to an enzymatic assay for glucose up to 10 mmol L−1 range. Practical utility of the developed bio-strips was confirmed by quantifying glucose in drinks and dietary supplement samples.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.