Abstract
Preparative isolation of deoxyriboadenylic acids from hydrolysates of oxidized herringsperm DNA using template-chromatography The alkaline hydrolysis of oxidized DNA from herring sperm yields a complex mixture of deoxyriboadenylic acids. After the removal of the fragments containing 1—3 monomer units by column chromatography, approximately 10% of the partial hydrolysate remains. This remaining fraction which contains the fragments of higher molecular weight is separated into two fractions by the base-pairing mechanism on a PV(pT) n —DEAE-Cellulose column with a two-step temperature gradient. The first fraction eluted at −4°, contains the substances (≈ 95% of the sample) that undergo no base-pairing with the immobilised oligothyidylic acid units. The remaining 5% of the sample that hybridizes with PV(pT) n —DEAE-Cellulose at −4°, is eluted at 30° (fraction 2). After enzymatic dephosphorylation, homologues of deoxyriboadenylic acid containing up to 8 monomer units are isolated on a preparative scale in chromatographically pure form from fraction 2 using column chromatography. Purity and structure of the isolated adenylic acids are determined by paper chromatography and by enzymatic hydrolysis.
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