Abstract
Transmissible spongiform encephalopathy (TSE) belongs to the group of contagious disease that affects the nervous system with fatal outcome. Chronic wasting disease belongs to this group and is characteristic for animals from the deer family. In this study polymorphisms in eleven codons located in exon 3 of the PrP gene has been investigated red deer (Cervus elaphus L.) population. Exon 3 regions, 628 bp long, were amplified by the PCR method from genomic DNA. Sequencing was performed by applying the Sanger dideoxi method. Results showed presence of eight different genotypes and eight different haplotypes. Susceptible genotypes were not found at a high frequency.
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