Abstract
A simple method for the proximate determination of triterpene glycosides in glycyrrhiza was developed. Glycyrrhiza extract was directly chromatographed on SP-Sephadex C-25 (H+) and QAE-Sephadex A-25 (Cl−) with distilled water and 0.2 M sodium chloride (pH 13.50) as the eluents, respectively. The eluate was then hydrolyzed with perchloric acid and determined turbidimetrically at 530 nm. With this method, glycyrrhiza samples collected in Taiwan and Singapore were found containing 17.21±0.45% and 7.49±0.35% of triterpene glycosides, calculated as glycyrrhizin, respectively.
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