Proximate Composition and Determination of Heavy Metal Content in Indian Fish using ICP-MS after Closed Vessel Micro Wave Digestion

Sreenivasa Rao J,Longvah T

doi:10.13189/fst.2016.040303

Abstract

The objective of the present study was to investigate the heavy metal content in fresh water fish collected from the outlets of twin cities of Hyderabad and Secunderabad, Andhra Pradesh, India. The heavy metal content of fresh water fish varieties was investigated using Inductively Coupled Plasma Mass Spectrophotometer (MD-ICP-MS) after microwave digestion. Heavy metal content in fresh water fish were ranged from 52-680 µg/kg for Nickel in Rastrelliger kanagurta and Stromateus sinensis, 38-690 µg/kg for Arsenic in Rastrelliger kanagurta and Pompus argenteus, 3-62 µg/kg for Cadmium in Masto symbollon and Pompus argenteus and 27-108 µg/kg for Lead in Panaeus monodon and Rastrelliger kanagurta respectively. Results were in accordance with recommended human daily intake values except for arsenic, which were in agreement with WHO/FAO recommended values. Since the available data for heavy metal content in fish is scanty, efforts were made to present a precise data for the same as estimated on ICP-MS. This study may provide information on important heavy metal concentrations of fresh water fish and shell fish and therefore provides an essential baseline data with which future levels may be compared and evaluated. Results were in accordance with recommended daily intake allowance by WHO/FAO.

Highlights

Fish and shellfish are one of the major sources of animal protein and have been widely accepted as a good source of nutrients for the maintenance of a healthy body
Among the varieties studied,(10-25cm and weight is 0.10-0.25 kg, 05–15 cm and weight is 0.10–0.20 kg, 15-35 cm weight is 0.50-3.5 kg).In the previous study conducted by Rejomon [19] the length of the fish and shell fish were gradually increased depending on Panaeus monodon and Stromateus sinensis with least length and weight (05–15 cm and weight is 0.05–0.15 kg) followed by Masto symbollon, Macrobrachium rosenbergii, and Rastrelliger kanagurta with highest length and weight the weight of the fish
Present study reveals that the moisture content of fresh water fish was diverse ranging from 70.19% in Arius sona and 78.39% in Cyprinus carpio

Summary

Introduction

Fish and shellfish are one of the major sources of animal protein and have been widely accepted as a good source of nutrients for the maintenance of a healthy body. These are increasingly marketed for their health benefits to consumers [1]. Water contamination is one of the serious concerns that affect the marine ecosystem with high concentration of trace metals. The metals accumulated in these waters infect the humans by direct consumption of water or through consuming the affected organisms like fishes [6,7] claim that when the level of trace metal concentrations exceeds the stipulated level; it turns out to be toxic. The work in Mc Lintock [8] has stated that the higher level of metal concentration will bring shattering effect to the ecological balance by altering the range of organisms in water

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Results

Conclusion