Abstract
The aim of this work was to order quantitative parameters of the proximal convoluted tubules (PCT) in the normal rat kidney cortex. Volume density (VV), both surface and specific surface density toward interstitium (SVi and SVi/VV) and toward lumen (SVl and SVl/VV) and thickness (T) of tubules epithelium have been stereologically ordered. Stereologically were analysed 170 test fields by lattice L36 on the paraffin sections of the three adult male Wistar rats kidney dyeing by PAS-method. The mean values of the variables analysed were: VV=76.4 % +/-0.012; SVi=0.056 microm-1 +/-0.004; SVl=0.028 microm-1 +/-0.003; SVi/VV=0.073 microm-1 +/-0.003; SVl/VV=0.037 microm-1 +/-0.005; T=18,26 microm +/-0.897. Stereological methods are making a very valuable contribution to science over recent years. We have used unbiased stereological counting methods to obtain objective quantitative parameters of the PCT epithelium in the normal rats' kidney cortex.
Highlights
The nephron is the fundamental structural and functional unit of the kidney
We calculated by means of standard stereological procedures volume density (VV) of the proximal convoluted tubules (PCT) epithelium, its surface density (SVl) and specific surface density (SVl/VV) toward lumen, its surface density (SVi) and specific surface density (SVi/VV) toward interstitium and its thickness (T)
The present results indicate that the useful information can be obtained about rat kidney by stereological investigation
Summary
The nephron is the fundamental structural and functional unit of the kidney. Each nephron consists of a dilated portion, the renal, or malpighian, corpuscle; the proximal convoluted tubule (PCT); the thin and thick portions of the loop of Henle; and the distal convoluted tubule (9). The proximal thick segment of the nephron begins as the PCT at Bowman's capsule; it makes a short turn toward the cortex and returns to its site of origin where it follows a very tortuous or convoluted course. Knowledge of normal quantitative parameters of PCT epithelium is very important and might be useful for comparison based on objective numerical values obtained in investigations of different experimental and pathological conditions. Stereological analyses were performed on normally structured tubular part of rat's kidney cortex to obtain some main numerical parameters of PCT epithelium. The aim of this work was to order quantitative parameters of the proximal convoluted tubules (PCT) in the normal rat kidney cortex. We have used unbiased stereological counting methods to obtain objective quantitative parameters of the PCT epithelium in the normal rats' kidney cortex
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