Abstract
The undulator beamline PROXIMA-1 at Synchrotron SOLEIL scheduled itsfirst users in March 2008. The endstation is dedicated to biomolecular crystallography experiments, with a layout designed to favour anomalous data recording and studies of crystals with large cell dimensions. In 12 years, the beamline has accommodated 4267 shifts of 8 h and more than 6300 visitors. By the end of 2020, it saw 1039 identified published scientific papers referring to1415 coordinates deposited in the Protein Data Bank. The current paper describes the PROXIMA-1 beamline, including the recent specific implementations developed for the sample environment. The setup installed in the experimental station contains numerous beam-shaping equipment, a chi-geometry three-axis goniometer, a single-photon-counting pixel-array X-ray detector, combined with a medium-throughput sample exchange robot. As part of a standard experimental scheme, PROXIMA-1 can also be accessed via `mail-in' services or remotely.
Highlights
Synchrotron SOLEIL is a 2.75 GeV French synchrotron radiation facility located on the Plateau de Saclay campus in the Paris suburbs
In the work by Poonsiri et al (2018), structures of the nonstructural protein 1 (NS1) from flavivirus solved at PROXIMA-1 were compared with smallangle scattering data recorded at the SAXS beamline SWING, a comparison that emphasized a number of differences in NS1 proteins and contributed to insightful information involving the relationships between the NS1 structures and their pathogenic functions towards the development of better diagnostics
The PROXIMA-1 beamline for macromolecular crystallography was developed at Synchrotron SOLEIL and has successfully served the crystallographic user community for over 12 years
Summary
Synchrotron SOLEIL is a 2.75 GeV French synchrotron radiation facility located on the Plateau de Saclay campus in the Paris suburbs. As of 2020, Synchrotron SOLEIL can accommodate 29 endstations, among which two beamlines are dedicated to macromolecular crystallography (MX) experiments. PROXIMA-1 was constructed around a user case for MX beamlines identified within the French community between 2000 and 2002. The beamline construction and the following improvements were executed while referring to a dedicated committee for MX studies. PROXIMA-1 observed its first beam at the sample position towards the end of 2007 and scheduled its first users in March 2008. The main purpose of PROXIMA-1, in the context of MX science, focuses on diffraction studies of large macromolecular assemblies with large lattices due to the low horizontal divergence of the beam at the sample. PROXIMA-1 serves academic research groups located in France (55%), Europe (38%) and worldwide
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