Abstract
Antimicrobial susceptibility testing (AST) should be conducted in a standardized manner prior to the start of an antimicrobial treatment. For fastidious bacteria, such as porcine Mycoplasma (‘Mesomycoplasma’) spp., specifically M. hyorhinis, neither guidelines or standards for the performance of AST, nor quality control strains for the validation of AST results are approved by organizations like the Clinical and Laboratory Standards Institute (CLSI) or the European Committee on Antimicrobial Susceptibility Testing (EUCAST). The CLSI- and EUCAST-approved quality control strains Enterococcus faecalis ATCC 29212 and Staphylococcus aureus ATCC 29213 were chosen to validate AST by broth microdilution using modified Friis broth, developed as growth medium for porcine Mycoplasma (‘Mesomycoplasma’) spp. The antimicrobial agents doxycycline, enrofloxacin, erythromycin, florfenicol, gentamicin, marbofloxacin, tetracycline, tiamulin, tilmicosin, tulathromycin, and tylosin were examined using customized SensititreTM microtiter plates. Minimal inhibitory concentrations, determined after 24, 48, and 72 h, were mostly within the CLSI-approved quality control ranges for defined antimicrobial agents. We propose the use of the combination of E. faecalis ATCC 29212 and S. aureus ATCC 29213 as surrogate quality control strains for the validation of future AST results obtained for M. hyorhinis by broth microdilution using modified Friis broth.
Highlights
Published: 28 August 2021Mycoplasma (‘Mesomycoplasma’) hyorhinis, first described by Switzer in 1955, is a widely distributed pathogen in pigs [1,2]
S. aureus ATCC 29213 as surrogate quality control strains for the validation of future antimicrobial susceptibility testing (AST) results obtained for M. hyorhinis by broth microdilution using modified Friis broth
ATCC 29213 are approved by Clinical and Laboratory Standards Institute (CLSI) and European Committee on Antimicrobial Susceptibility Testing (EUCAST), alongside available quality control (QC) ranges in cationadjusted Mueller-Hinton broth
Summary
Mycoplasma (‘Mesomycoplasma’) hyorhinis, first described by Switzer in 1955, is a widely distributed pathogen in pigs [1,2]. Spp., is especially suited for culturing these fastidious organisms To our knowledge, this complex broth medium best provides the needed nutrients for porcine Mycoplasma (‘Mesomycoplasma’) spp. The modified Friis broth used in this study, based on the recommendation of the German Collection of Microorganisms and Cell Cultures GmbH (DSMZ, Braunschweig, Germany) for culturing porcine Mycoplasma (‘Mesomycoplasma’) spp. is—compared to the original Friis broth—free of antimicrobial agents and better suited for AST [8]. ATCC 29213 are approved by CLSI and EUCAST, alongside available QC ranges in cationadjusted Mueller-Hinton broth We tested these two QC strains for their performance in modified Friis medium and propose the provisional combinational use of these two QC strains as surrogate quality controls until a M. hyorhinis QC strain is approved
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