Abstract
Somatic cell hybrids between an AKR mouse thymic lymphoma cell line and normal F344 rat spleen cells were isolated in order to assign the rat major histocompatibility complex (MHC) (RT1) to a specific chromosome. Six primary hybrid clones and 3 of their derivatives were examined for expression of the RT1 antigens on the cell surface by the complement-dependent cytotoxicity test and simultaneously for their karyotypes by the Hoechst 33258/quinacrine mustard double staining method. All of these hybrid clones consisted of 2 sets of mouse and 1 set of rat parental cells, with a preferential loss of rat chromosomes. A high degree of concordance was shown between the expression of the RT1 antigen and the presence or absence of rat chromosome 14 in the original 6 hybrid clones. This was confirmed in 1 spontaneous and 2 immunoselected RT1-negative segregants from RT1-positive clones. These results led us to propose that the rat MHC is located on chromosome 14.
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