Abstract
Pathogenesis of retinal capillary hemangioma has not been sufficiently studied at the present time. Therefore, the study of cytokine levels in biological fluids seems to be very relevant in order to increase knowledge about the mechanisms of the disease development and searching for targeted therapies. The content of hematopoietic and vasoactive growth factors in blood serum, lacrimal fluid, and vitreous body was studied in patients with retinal capillary hemangioma. A total of 26 patients with retinal angiomatosis were examined. The samples of blood serum (n = 23) and lacrimal fluid (n = 10) from practically healthy people aged 22 to 46 (27.4±1.4 years) were used as a control. To perform comparative assessment of cytokine concentrations in the vitreous body of patients with retinal capillary hemangioma, were used samples of the vitreous body from 6 patients (average age 33±4.7 years; from 21 to 49 years) with rhegmatogenous retinal detachment. To measure the cytokine concentrations, we applied multiplex analysis technique using the xMAP platform with LuminexxPONENT 3.1 program and ProcartaPlex sets (eBioscience, Austria). A detailed characteristic of vasoactive factors in capillary retinal hemangioma was obtained as a result of this work. Some disorders in chemokine regulation were identified. There was a significant increase in serum concentrations of three vasoactive factors, i.e., PDGF-BB, HGF, and PIGF-1, with a decrease in chemokines (MCP-1, MIP-1α, and MIP-1β). The frequencies of PIGF-1 and MIP-1α detection also significantly differed from the control group. SCF was significantly more often determined in patients with retinal angiomatosis only at the systemic level. Correlations between PDGF-BB and PIGF-1, as well as PIGF-1 and MIP-1β were shown. A significant increase in VEGF-A, HGF, VEGF-D, as well as MCP-1 concentrations was shown in the lacrimal fluid. The inversion of PDGF-BB concentrations in serum and lacrimal fluid was noted. Analysis of intraocular cytokine levels revealed a significant increase in VEGF-A and HGF concentrations, with marked decrease in MIP-1α and MIP-1β. PDGF-BB in 100% of cases was determined only in vitreous body of patients with retinal angiomatosis. With respect to the revealed characteristic shifts of HGF/SF intraocular production in retinal capillary hemangioma, it seems relevant to search ways for its inhibition, thus providing potential basis for a new therapeutic strategy in treatment of retinal angiomatosis.
Highlights
Факторы роста при ангиоматозе сетчатки Growth factors in retinal angiomatosis возникает в результате мутаций в гене-супрессоре опухолевого роста VHL и ассоциирован с развитием целого ряда доброкачественных и злокачественных опухолей, а также кист различных локализаций [17, 49]
Поэтому разработка подходов к ранней диагностике ангиоматоза сетчатки является актуальной задачей, так как позволит сохранить зрение и обеспечить пациентам высокое качество жизни
Wang Y., Abu-Asab M.S., Shen D., Zhuang Z., Chew E.Y., Chan C.C. Upregulation of hypoxia-inducible factors and autophagy in von Hippel–Lindau-associated retinal hemangioblastoma
Summary
Нероев В.В., Балацкая Н.В., Новикова А.Ю., Рябина М.В., Илюхин П.А. В представленном исследовании изучено содержание в сыворотке крови, слезной жидкости и стекловидном теле гемопоэтических и вазоактивных факторов роста у пациентов с капиллярной гемангиомой сетчатки. В сыворотке крови было выявлено достоверное увеличение концентраций 3 вазоактивных факторов – PDGF-BB, HGF и PIGF-1, при снижении хемокинов – МСР-1, MIP-1α и MIP-1β. Анализ внутриглазных уровней цитокинов выявил достоверное увеличение концентраций VEGF-A и HGF, при выраженном снижении MIP-1α и MIP-1β. Фактор роста PDGF-BB в 100% случаев определялся только в стекловидном теле пациентов с ангиоматозом сетчатки. Илюхин «Известные и малоизученные гемопоэтические и вазоактивные факторы роста при капиллярной гемангиоме сетчатки» // Медицинская иммунология, 2020. Медицинская Иммунология Medical Immunology (Russia)/Meditsinskaya Immunologiya интраокулярной продукции HGF/SF при капиллярной гемангиоме сетчатки, представляется актуальным поиск способов его ингибирования, что может стать основой новой терапевтической стратегии в лечении ангиоматоза сетчатки. Helmholtz National Medical Research Center of Eye Diseases, Moscow, Russian Federation
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