Abstract
PROTOPORPHYRIN PRECURSORS PRODUCED BY A CHLORELLA MUTANT
Highlights
From the optical density of a porphyrin solution extracted from an aliquot of the brown material, that the yield per bottle is about 1.0 gm. of porphyrin mixture
From the intensity of the spots on the paper it was evident that the more abundant porphyrins were those containing two carboxyl groups; these appeared in each fraction of HCl
A Chlorella mutant, WbB-17, has been isolated that produces a mixture of pigments which, on the basis of the Nicholas-Rimington method, appear to represent porphyrins containing two, three, four, five, six, seven, and eight carboxyl groups
Summary
50 Blake bottles containing a liter of solid agar medium on which the Chlorella had grown were added 500 cc. The somewhat dry material on the filter paper (about 100 gm.) was stirred with a solvent made up of 98 volumes of acetic acid and 2 volumes of concentrated HCl and extracted by centrifuging three times with a total volume of 1.5 liters of this solvent. There remained a small amount of pigment in the ether not extractable by 1 N HCl. The main ether solution contained the porphyrins from the cells and the porphyrins which had diffused from the cells into the liquid medium. Likewise the porphyrins which were not readily soluble in ether and had to be adsorbed on fullers’ earth from aqueous solution included porphyrins containing seven and eight carboxyl groups. From the intensity of the spots on the paper it was evident that the more abundant porphyrins were those containing two carboxyl groups; these appeared in each fraction of HCl
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