Abstract
Haploid microspore-derived plants from amphidiploid Brassica napus give rise in culture to very large numbers of secondary stem embryoids from single epidermal cells. The embryoids can be excised, transferred to fresh medium and induced to repeat the process 4–6 times. Eventually the embryoids form larger plantlets which can be grown to maturity. Spontaneous amphihaploid plants or microspore-derived plants growing either in vitro or in vivo have been used as a source of plant protoplasts. Such protoplasts have been induced to produce calluses capable of both root and shoot production. The limited number of plantlets so far obtained from large numbers of calluses have retained their haploidy. The significance of haploid single-cell cultures of such agriculturally important fodder crops is discussed.
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