Abstract
Sweet potato (Ipomoea batatas L. Lam) ranks second in world wide root and tuber production. Loss of genetic resources in the primary centers of origin have been occurring for a number of years. Utilization of the genetic diversity of wild types and related species cart expand breeding potential and efficiency. Two species, Ipomoea trifida and Ipomoea triloba, have been identified as possible progenitor species or sweet potato. Plant improvement at the cellular level using protoplasts is a novel alternative to conventional breeding practices. Protoplast plating efficiency appears to be genotype dependent. Roots have been regenerated from Ipomoea trifida protoplasm calli on Murashige and Skoog's medium containing zeatin or kinetin.
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