Abstract

The first steps to successful microinjection in plant cells of different types of macromolecules (RNA, DNA and protein) are reported. Evidence is also given that plant cells can survive the mechanical injury of the injection procedure. By attaching the cells to coverslips with polylysine, the progeny of an individual cell can be followed microscopically. Using the fluorescent dye Lucifer Yellow, the injection procedure can be monitored and the injected volume calculated.

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