Abstract
The accompanying communication [Eur. J. Biochem. 141 (1984) 1-4] indicates that the microinjection of the pH fluorescent indicator pyranine (8-hydroxy-1,2,6- pyrenesulfonate ) into giant mitochondria or mitoplasts does not affect their ability to carry out oxidative phosphorylation. The dye can therefore be used as a quantitative indicator of internal mitochondrial pH. We found that activation of metabolism in rotenone-inhibited giant mitochondria by the addition of succinate produces an internal pH change corresponding to a pH shift of 0.3 to the alkaline range, approximately the same value found previously for conventional rat liver mitochondria.
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