Abstract
Combined effect of vanadium and beta-carotene on rat liver DNA-chain break and Proton induced X-ray emission (PIXE) analysis was studied during a necrogenic dose (200 mg/kg of body weight) of Diethyl Nitrosamine (DENA) induced rat liver carcinogenesis. Morphological and histopathological changes were observed as an end point biomarker. Supplementation of vanadium (0.5 ppm ad libitum) in drinking water and beta-carotene in the basal diet (120 mg/Kg of body weight) were performed four weeks before DENA treatment and continued till the end of the experiment (16 weeks). PIXE analysis revealed the restoration of near normal value of zinc, copper, and iron, which were substantially altered when compared to carcinogen treated groups. Supplementation of both vanadium and beta-carotene four weeks before DENA injection was found to offer significant (64.73%, P < 0.001) protection against generation of single-strand breaks when compared with the carcinogen control counter parts. A significant stabilization of hepatic architecture of the cells was observed as compared to carcinogen control in vanadium plus beta-carotene treated group. This study thus suggests that vanadium, a prooxidant but potential therapeutic agent yield safe and effective pharmacological formulation with beta-carotene, an antioxidant, in the inhibition of experimental rat hepatocarcinogenesis.
Highlights
∇ DENA injection (200 mg/ kg bw. i.p) Treatment with Vanadium (0.5 ppm) in drinking water ad libitum daily Treatment with Beta-carotene (120 mg/kg bw six days a week) in the basal diet
⇓ Rats sacrificed for DNA chain Break analysis 18-20h after DEN injection Animals sacrificed for PIXE analysis and Histology
Summary
∇ DENA injection (200 mg/ kg bw. i.p) Treatment with Vanadium (0.5 ppm) in drinking water ad libitum daily Treatment with Beta-carotene (120 mg/kg bw six days a week) in the basal diet.
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