Abstract

The ORD and CD spectra and fluorescence measurements of complexes between poly(I) and poly(C) at different pH and ionic strength are reported. The evolution of optical activity and fluorescence closely follows the results obtained earlier on this system by spectrophotometric methods. These data clearly distinguish three different complexes: poly(I) · poly(C), poly(I) · poly(C) · poly(C +) and poly(I) · poly(C +). The latter is probably a reverse Hoogsteen paired helix. Comparison with published data on poly(dI) · poly(dC) suggest that the deoxypolymer may be a non-Watson-Crick helix.

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