Abstract
The aim of this work was to assess the effect of chronic administration of protonated nanostructured aluminosilicate (NSAS) on the plasma cholesterol levels and development of atherosclerotic lesions in Apolipoprotein (ApoE) deficient mice fed a high cholesterol and high fat diet. Apolipoprotein E (ApoE) deficient mice were divided into the following treatment groups: protonated NSAS 1.4% (w/w), untreated control and 2% (w/w) stigmastanol mixed with high-cholesterol/high-fat diet. Animals were treated for 12 weeks, blood samples were withdrawn every 4 weeks for determination of plasma cholesterol and triglyceride levels. At the end of the study the aortic roots were harvested for assessment of atherosclerotic lesions. NSAS at 1.4% (w/w) and stigmastanol at 2% (w/w) treatment groups showed significant decreases in plasma cholesterol concentrations at all time points relative to the control animals. The lesion sum area in 1.4% (w/w) NSAS and 2% (w/w) stigmastanol groups were significantly less from the control animals. In conclusion, in this study, the effectiveness of chronic administration of protonated NSAS material in the reduction of plasma cholesterol levels and decrease in development of atherosclerotic lesions was demonstrated in Apo-E deficient mice model.
Highlights
Elevated plasma cholesterol levels have been associated with increased risk of atherosclerosis and coronary artery disease [1]
The current study was designed to elucidate the effect of chronic administration of protonated form of nanostructured aluminosilicate (NSAS) to Apolipoprotein-E (Apo-E) deficient mice on plasma cholesterol concentrations and formation of aortic atherosclerotic lesions
The treatment by protonated NSAS at 1.4% w/w and stigmastanol at 2% w/w resulted in a significant decrease in plasma cholesterol levels at 4, 8 and 12 weeks of the study compared to untreated controls
Summary
Elevated plasma cholesterol levels have been associated with increased risk of atherosclerosis and coronary artery disease [1]. The main agents are plant sterols, plant stanols and ezetimibe Since these agents are reported to be absorbed into blood circulation, there is a potential for systemic adverse effects [2,3,4,5]. We have previously reported that protonated nanoscaled aluminosilicate compound efficiently inhibited the intestinal absorption of cholesterol following acute administration in a rat model [[9]; See Additional File 1]. It was unclear, what will be the effect of NSAS in the chronic administration on plasma cholesterol levels and on development of pathophysiology of atherosclerosis [10]. The current study was designed to elucidate the effect of chronic administration of protonated form of NSAS to Apolipoprotein-E (Apo-E) deficient mice on plasma cholesterol concentrations and formation of aortic atherosclerotic lesions
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