Abstract
With 1,3-dimethyluracil and 1,3,6-trimethyluracil as models for nucleic acid bases, it was found by infrared spectroscopic methods that the site of protonation is a carbonyl group. Analysis of other evidence makes it likely that it is the carbonyl group in position 4. In 1,3,6-trimethyluracil salts, activation of the 5 position under acid conditions permits ready hydrogen–deuterium exchange.Some anomalous 2 base – 1 acid salts with complex acids are considered in the light of base pairing errors in nucleic acids.
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