Abstract
Molecular motion and molecular organization of human serum low-density lipoprotein (LDL) has been studied in the temperature range − 30 to 30°C by proton magnetic relaxation. LDL in deuterated Tris-HCl buffer exhibit two mobile phases. The slow-relaxing phase ( T 1 ⋍ 1.5 s ) is assigned to the incompletely deuterated water of the buffer, and the fast-relaxing phase ( T 1 ⋍ 60 ms ) to the fatty acid chains of the lipoprotein core. It has been established that there is a correlation between the state of the outer surface and the interior of the LDL particle: the number of fast-relaxing protons is significantly altered by cooling the system through the freezing point of the buffer or by selecting buffers of different ionic strengths. At room temperature, ∼ 30% of the lipid protons of LDL in the 0.1 m buffer and ∼ 40% of the lipid protons of LDL in the 0.01 m buffer relax quickly within the time-scale of n.m.r. frequency (24 MHz).
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