Protocols in Molecular Neurobiology

Abstract

Separation of Large DNA Molecules by Pulsed-Field Gel Electrophoresis. The Isolation of Genomic DNA from Invertebrates. Analysis of Mitochondrial DNA Mutations. The Design and Use of Oligonucleotides. The Use of Degenerate Oligonucleotides for Polymerase Chain-Reaction-Based Isolation of Related DNA Sequences. Isolation of Photoreceptor Cell-Specific MEKA cDNA by Differential Hybridization. Generation of Isoform-Specific Antisera from Cloned cDNAs: Application to Multiple Forms of the Na/K-ATPase Expressed in Rat Brain. Determination of Transcriptional Initiation Sites and Their Usage in the Nervous System. Mapping of Trans-Acting Factor Binding in the Nervous System. In Situ Hybridization to Brain Tissue Sections Using Labeled Single-Strand Complementary RNA Probes. In Situ Hybridization Histochemistry Using Alkaline Phosphatase- Labeled Oligodeoxynucleotide Probe. The Identification of Neuropeptide Gene Regulatory Elements in Transgenic Mice. Expression of Exogenous Ion Channels and Neurotransmitter Receptors in RNA- Injected Xenopus Oocytes. Analysis of Insulin and Insulin-Like Growth Factor-I Receptors in Neural Tissues. Use of Affinity Chromatography in Purification of A1 Adenosine Receptors from Rat Brain Membranes. Purification and Structure of L-Type Calcium Channels. Purification and Reconstitution of the Ryanodine-Sensitive Ca2+ Release Channel Complex from Muscle Sarcoplasmic Reticulum. Identification of a Ligand-Gated Ion Channel by Photoaffinity Labeling and Microsequencing. Voltage-Gated Ion Channels: Electrophysiological Approaches. An Electrophysiological Approach to the Regulation of Neuronal Voltage-Activated Calcium Channels by Guanine Nucleotide Binding Proteins. Index.