Year-end Sale % OFF 
Abstract
The protocol of refinement in neutron protein crystallographic analysis is described in this article. During the refinement, there are several features specialized for neutron experiment:(1) the mixed chemical structure of deuterium and hydrogen atoms, (2) solvent molecules around the protein, and the deuterium exchange in the polypeptide chain. We demonstrate the example of neutron refinement steps, using rubredoxin mutant, of which neutron diffraction collected using BIX-3 diffractometer at JRR-3 in Japan Atomic Energy Agency. The X-ray refinement program of CNS was used for the present refinement. Scattering lengths, stereochemical parameters, and command input files were modified for neutron experiment. These files would be the templates for the neutron protein crystallographic analysis.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
