Abstract
This protocol is a more detailed version of previous protocols (Yang et al., 2011; Bolaños-Villegas et al., 2013) developed for the examination of meiotic chromosome spreads. Meiotic chromosome spreads are useful to determine the presence of defects in chromosome pairing and segregation. The protocol also describes how to perform fluorescent in situ hybridization experiments with a centromere probe used to label chromosomes., 该方案是为研究减数分裂染色体扩散而开发的以前方案的更详细的版本(Yang等人,2011;Bolaños-Villegas等人,2013)。 减数分裂染色体扩散可用于确定染色体配对和分离中缺陷的存在。 该方案还描述了如何使用用于标记染色体的着丝粒探针进行荧光原位杂交实验。
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have