Abstract
Dragon's blood, native species from South America, has therapeutic properties scientifically proved. This study had the objective of developing a protocol for in vitro establishment and plantlets acclimatization from zygotic embryos, since researches with species are incipient. Culture media (MS, WPM, QL and N6) were assessed, without and with activated charcoal (2 g L-1). It was observed the percentage of developed embryos from 83% to 100% and the effect of medium composition for plant height and leaf number in vitro. The plantlets were acclimatized with 100% of survival rate, and for treatments from in vitro culture only the culture medium influenced diameter and plant height. The WPM and QL formulations without activated charcoal are indicated for in vitro culture of embryos and acclimatization of Dragon blood. The suggested methodology represents an important strategy for reproduction, physiological study and preservation of species.
Highlights
Considering the importance of Croton lechleri on the treatment of multiple symptomatologies, added to the predatory means of latex attainment; the objective of this study was to develop a protocol for in vitro establishment and acclimatization of C. lechleri plants originated from zygotic embryos
The research was fulfilled at the Biotechnology and Plant Anatomy Laboratory and at the Experimental Area of Universidade Federal do Acre (UFAC), Rio Branco, active charcoal (AC)
Every embryo labeled as developed resulted in a normal plant, with a survival rate of 100%
Summary
The cultivation of tissues is applied and suggested (Morais et al, 2012; Oliveira et al, 2011) envisioning the production of certified and standardized raw material, free of phytopathogens and plague disseminated by conventional propagation methods (Mafia et al, 2012). Such aspects are crucial for clonal cleaning and conservation in vitro of exotic and native species (Machado et al, 2013). Apart from reducing the time required for the attainment of new healthy specimens, it guarantees elevated development of in vitro embryos (Ebert et al, 2014) and allows for studies related to the embryo’s physiology (Haslam & Yeung, 2011)
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