Abstract
A trans-ferulic acid-utilizing Pseudomonas sp. HF-1 was isolated from soil samples. Mutant HF-1124, capable of growing on trans-ferulic acid but not on protocatechuic acid, was isolated from HF-1 after mutagenesis with nitrosoguanidine. The optimum temperature was 30°C and the optimum pH was 7.0–8.0 for protocatechuic acid production from trans-ferulic acid by mutant HF-1124. Protocatechuic acid production reached 4 g/l from a concentration of 8 g/l trans-ferulic acid. As a result of co-oxidation of methoxy aromatic compounds by strain HF-1124 grown on acetic acid, protocatechuic acid was formed from vanillin and vanillic acid, and vanillic acid and isovanillic acid were formed from veratric acid. By the co-oxidative demethylation of substituted monomethoxybenzene, m- and p-hydroxybenzoic acids were accumulated from m-and p-anisic acid, respectively, while no products were detected from anisole, o-anisic acid, nitroanisole, methylanisole, methoxyphenol and dimethoxybenzene.
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