Abstract

Seafood is considered one of the main food allergen sources by the European Food Safety Authority (EFSA). It comprises several distinct groups of edible aquatic animals, including fish and shellfish, such as crustacean and mollusks. Recently, the EFSA recognized the high risk of food allergy over the world and established the necessity of developing new methodologies for its control. Consequently, accurate, sensitive, and fast detection methods for seafood allergy control and detection in food products are highly recommended. In this work, we present a comprehensive review of the applications of the proteomics methodologies for the detection and quantification of seafood allergens. For this purpose, two consecutive proteomics strategies (discovery and targeted proteomics) that are applied to the study and control of seafood allergies are reviewed in detail. In addition, future directions and new perspectives are also provided.

Highlights

  • Changes in life habits, including food production and manufacturing, have dictated a global increase in adverse food reactions [1,2,3]

  • The in vivo diagnosis of a shellfish allergy using skin prick tests (SPT) still requires the use of home-made fresh extracts given the rather low number of commercially available extracts compared to the offending species, as well as their differences in allergen content [95]

  • The development and use of proteomic approaches have undoubtedly played a key role in the identification, detection, and quantification of seafood allergens

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Summary

Introduction

Changes in life habits, including food production and manufacturing, have dictated a global increase in adverse food reactions [1,2,3]. Among these reactions, type I IgE-mediated allergies to food components are considered by the World Health Organization (WHO) as the fourth most important public health problem. Type I IgE-mediated allergies to food components are considered by the World Health Organization (WHO) as the fourth most important public health problem These food allergies affect an estimated 6–8% of young children and 2–4% of adults with regional variations [3].

Seafood
Proteomic Workflows
Proteomics Applications in the Diagnosis of Seafood Allergy
Concluding Remarks and Future Directions
Findings
Methods
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