Abstract
Wheat (Triticum aestivum), as a kind of important economic crop cultured in the Northern China, is affected by present-day enhanced ultraviolet-B (UV-B) radiation. To get the information of the impact by UV-B radiation on it, the proteins of wheat (Jin mai NO.8) leaves, which were divided into the normal light group (CK) and UV-B radiation group (B), were extracted and ran at SDS-PAGE at different treatment days (5, 6, 7). The proteins were also analyzed by run two-dimensional gel electrophoresis (2-DE), which allowed the identification of some significantly different gel spots. The proteins spots were further verified by Matrix-Assisted Laser Desorption/lonization-time of Flight Mass Spectrometry. The results showed: 1) the enhanced UV-B affects the growth of the wheat, as the visual changes appear on the sixth day; 2) the proteins expressions between the B group and the CK group were remarkably different on the sixth day; 3) the proteins of wheat leaves of the sixth day were further analyzed by 2-DE revealed that twenty-one protein points were identificated between the B group and the CK group. Among these twenty-one proteins, six proteins of them were up-regulated and twelve proteins of them were down-regulated, three new proteins were expressed only in the B group. Three proteins among six proteins, which were up-regulated, were further verified as RuBisCo large subunit binding protein; SOD; Calmodulin. The result indicates wheat could improve genes encoding proteins in their leaves and protect themselves, when enhanced UV-B affects the growth of the wheat.
Highlights
With reduction of stratospheric ozone increases ultraviolet-B (UV-B) radiation at the earth’s surface, UV-B radiation has been shown to be harmful to plants [1]
The results showed: 1) the enhanced UV-B affects the growth of the wheat, as the visual changes appear on the sixth day; 2) the proteins expressions between the B group and the CK group were remarkably different on the sixth day; 3) the proteins of wheat leaves of the sixth day were further analyzed by 2-DE revealed that twenty-one protein points were identificated between the B group and the CK group
The result indicates wheat could improve genes encoding proteins in their leaves and protect themselves, when enhanced UV-B affects the growth of the wheat
Summary
With reduction of stratospheric ozone increases ultraviolet-B (UV-B) radiation at the earth’s surface, UV-B radiation has been shown to be harmful to plants [1]. UV-B can reduce plant growth and has the potential to jeopardize the functionality of cellular components, such as DNA, protein [2]. Most of the studies suggest that a range of characteristics such as gene expression for growth and development, Physiological changes could increase the ability of the plants to cope with UV-B radiation [3,4]. As the leaves could assimilate carbon for plants filling during the ripening phase, and lead to the identification of candidate regulatory proteins. Tool for the analysis of gene expression of wheat leaves in response to UV-B radiation. How UV-B radiation is transformed into biomass wheat leaves is the most important issue. The response of wheat leaves under UV-B exposure has been observed
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
