Abstract
Abstract RIG-I-like receptors (RLRs) and Toll-Like Receptors (TLRs) play a critical role in sensing microorganisms and activating the early innate immune response. The signaling circuit of innate immunity remains incompletely understood. To systematically investigate the network, we initiated a global proteomic analysis of protein complexes found in the innate immunity pathway. Fifty-five genes involved in TLR and RLR pathways were tagged with FLAG epitopes and stably transfected into 293 cells engineered to express TLR3 or TLR4. After treatment with or without poly(I:C), LPS, or poly(dA:dT), cells were collected to purify the tagged protein complexes. Proteins in each complex were identified by mass spectrometry and data were statistically analyzed. Protein interaction networks were established using Cytoscape software. Sixty-five known interactions were confirmed and seventy-nine new interactions were detected.
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