Abstract
Brucellae are intracellular sneaky bacteria and they can elude the host’s defensive mechanisms, resulting in therapeutic failure. Therefore, the goal of this investigation was to rapid identification of Brucella species collected from animals and humans in Saudi Arabia, as well as to evaluate their resistance to antibiotics. On selective media, 364 animal samples as well as 70 human blood samples were cultured. Serological and biochemical approaches were initially used to identify a total of 25 probable cultured isolates. The proteomics of Brucella species were identified using the MALDI Biotyper (MBT) system, which was subsequently verified using real-time polymerase chain reaction (real-time PCR) and microfluidic electrophoresis assays. Both Brucella melitensis (B. melitensis) and Brucella abortus (B. abortus) were tested for antimicrobial susceptibility using Kirby Bauer method and the E-test. In total, 25 samples were positive for Brucella and included 11 B. melitensis and 14 B. abortus isolates. Twenty-two out of 25 (88%) and 24/25 (96%) of Brucella strains were recognized through the Vitek 2 Compact system. While MBT was magnificently identified 100% of the strains at the species level with a score value more than or equal to 2.00. Trimethoprim-sulfamethoxazole, rifampin, ampicillin-sulbactam, and ampicillin resistance in B. melitensis was 36.36%, 31.82%, 27.27%, and 22.70%, respectively. Rifampin, trimethoprim-sulfamethoxazole, ampicillin, and ampicillin-sulbactam resistance was found in 35.71%, 32.14%, 32.14%, and 28.57% of B. abortus isolates, correspondingly. MBT confirmed by microfluidic electrophoresis is a successful approach for identifying Brucella species at the species level. The resistance of B. melitensis and B. abortus to various antibiotics should be investigated in future studies.
Highlights
Brucellosis is a prevalent zoonotic disease that continues to be community health obstacles around the world [1]
B. melitensis, which was first isolated from sheep, goats, and camels; B. abortus of cows; Brucella suis (B. suis) of pigs; and Brucella canis (B. canis) of canines are all virulent to humans [4]
Modified Ziehl-Neelsen (MZN) stain was applied for all isolates to demonstrate their
Summary
Brucellosis is a prevalent zoonotic disease that continues to be community health obstacles around the world [1]. B. melitensis is one of the most prevalent aggressive and pathogenic species of Brucella, causing the most serious sickness [6]. B. abortus, on the other hand, is the least pathogenic species and causes the sickness to be mild. B. melitensis is often regarded as the primary cause of brucellosis in humans around the world. Despite being under control in several affluent nations, the disease stills prevalent in the Kingdom of Saudi Arabia, where the disease has a nationwide sero-prevalence of about 15% [7] This disease is brought into Saudi Arabia via the unrestricted importation of various animals that have not been thoroughly checked for the disease [7, 8]
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