Abstract
Proteomics study was performed to investigate the specific protein expression profiles of HepG2 cells transfected with mutant HBV compared with wildtype HBV genome, aiming to identify the specific functions of SH3 binding domain (proline rich region) located in HBx. In addition to the cell movement and kinetics changes due to the expression of HBV genome we have observed previously, here we further targeted to explore the specific changes of cellular proteins and potential intracellular protein interactions, which might provide more information of the potential cellular mechanism of the differentiated cell movements. Specific changes of a number of proteins were shown in global protein profiling in HepG2 cells expressing wildtype HBV, including cell migration related proteins, and interestingly the changes were found recovered by SH3 binding domain mutated HBV. The distinctive expressions of proteins were validated by Western blot analysis.
Highlights
Hepatocellular carcinoma (HCC) is the third fatal reason of cancer related death in the world
The proteins appeared in at least three independent repeats with consistent trends of changes for both of transfected cell lines when compared with control, which was wildtype HBV (wHBV)/ctrl, or mutated HBV (mHBV)/ctrl, were considered in the following analysis and discussion
17 proteins were considered as significantly changed and listed here (Table 1). These proteins were found perturbed by the expression of wildtype HBV genome, the changes were recovered in different distances by the mutation of proline rich region in HBx
Summary
Hepatocellular carcinoma (HCC) is the third fatal reason of cancer related death in the world. HBV infection, due to the high prevalence compared with HCV, outweighs HCV as the most clinico-epidemiologically important risk factor of HCC [2]. Hepatitis B virus genome is circular and partially double stranded DNA It is composed of around 3200 base pairs with slight differences among the eight genotypes [3]. HBx has been well accepted as a multifunctional gene and plays an important role in the development of HBV related liver diseases. It has been reported as transcriptional regulator and it is believed to participate in many intracellular signal pathways, such as signal transduction, apoptosis, cell cycle progression and protein degradation pathways through interaction with host genes. Numerous work has been done in many groups in the past years, the knowledge of HBx is not comprehensive, especially the specific molecular mechanism through which HBx interacts with host cell factors
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
