Abstract
BackgroundCaprine parainfluenza virus type 3 (CPIV3) is major pathogen of goat herds causing serious respiratory tract disease and economic losses to the goat industry in China. We analyzed the differential proteomics of CPIV3-infected Madin-Darby bovine kidney (MDBK) cells using quantitative iTRAQ coupled LC-MS/MS. In addition, four DEPs were validated by qRT-PCR and western blot analysis.ResultsQuantitative proteomics analysis revealed 163 differentially expressed proteins (DEPs) between CPIV3-infected and mock-infected groups (p-value < 0.05 and fold change > 1.2), among which 91 were down-regulated and 72 were up-regulated. Gene ontology (GO) analysis showed that these DEPs were involved in molecular functions, cellular components and biological processes. Biological functions in which the DEPs were involved in included diseases, genetic information processing, metabolism, environmental information processing, cellular processes, and organismal systems. STRING analysis revealed that four heat shock proteins (HSPs) included HSPA5, HSPA1B, HSP90B1 and HSPA6 may be associated with proliferation of CPIV3 in MDBK cells. qRT-PCR and western blot analysis showed that the selected HSPs were identical to the quantitative proteomics data.ConclusionTo our knowledge, this is the first report of the proteomic changes in MDBK cells after CPIV3 infection.
Highlights
Caprine parainfluenza virus type 3 (CPIV3) is major pathogen of goat herds causing serious respiratory tract disease and economic losses to the goat industry in China
Another study reported that 35% of nasal swabs and serum samples from clinically diseased goats were positive for CPIV3 by quantitative RT-PCR [5]
CPIV3 propagation in Madin-Darby bovine kidney (MDBK) cells The kinetics of CPIV3 propagation in MDBK cells were observed by monitoring the cytopathic effect (CPE) at 24, 48 and 72 h post infection (Fig. 1a), a minimal CPE was visible at 24 hpi, whereas an obvious CPE was observed at 48 hpi, and at 72hpi, almost all cells were disrupted
Summary
Caprine parainfluenza virus type 3 (CPIV3) is major pathogen of goat herds causing serious respiratory tract disease and economic losses to the goat industry in China. Genome sequence alignment and phylogenetic analysis revealed that the genome of CPIV3 strain JS2013 showed only. This pathogen was designated as CPIV3, a member of the PIV3 group belonging to the Respirovirus genus within the Paramyxiviridae family. A seroprevalence study using 2919 serum samples in China reported a CPIV3 prevalence of 39.9% in goats [4]. Another study reported that 35% of nasal swabs and serum samples from clinically diseased goats were positive for CPIV3 by quantitative RT-PCR (qRT-PCR) [5]. It is noteworthy that the spread of CPIV3 has caused heavy economic losses in China [6]
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