Abstract
：Objective We usedproteomic profiling in an attempt to differentiate and identify histological proteins thatwere associated with atherosclerosis obliterans (ASO) of human femoral artery.Methods Wecomparatively analyzed the proteome of 8 atherosclerotic and 5 normal femoral arteries.The differentially expressed proteins were visualized by two dimensional electrophoresis(2-DE) and sequenced by matrix assisted laser desorption/ionization mass spectrometry(MALDI-TOF-MS). The protein identification program was used to search the National Centerfor Biotechnology Information (NCBI) database and International Protein Index (IPI).Results A total of 53 distinct spots corresponding to 13 different proteins wereidentified by MALDI-TOF-MS using the NCBI and IPI databases. The function information ofthese 13 proteins mainly involved the pathogenetic mechanisms such as inflammation, innateimmunity, oxidative stress, lipid metabolism, amyloid degeneration and so on. ConclusionASO is associated with distinct patterns of protein expression in the femoral arteries,and differentially expressed 13 proteins may contribute to the pathogenesis. Thesefindings might provide needed biomarkers for ASO and new insight into its pathophysiology.
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