Proteomics analysis of the protective effect of canola (Brassica campestris L.) bee pollen flavonoids on the tert-butyl hydroperoxide-induced EA.hy926 cell injury model

Abstract

• Canola bee pollen flavonoids can protect against vascular endothelial cell injury. • Canola bee pollen flavonoids have antioxidant and anti-inflammatory effects on EA.hy926 cells. • iTRAQ proteomics technology was used for protection mechanism analysis. • The mechanism involves regulation of oxidative stress, lipid metabolism and mitochondrial function. Pollen contains various antioxidants, including flavonoids, which are beneficial to cardiovascular health. However, the protective effect and mechanism of pollen flavonoids extract on the cardiovascular system are still unclear. Herein, the protective effects of crude flavonoids of pollen (CFP) from canola ( Brassica campestris L. ) on tert-butyl hydroperoxide (TBHP)-induced cell injury were assessed in the EA.hy926 cell line. Then, the potential mechanism by which canola CFP protects vascular endothelial cells was explored at the proteome level and was verified by determining relevant indicators. Canola CFP exerted antioxidant and anti-inflammatory effects on the EA.hy926 cell injury model. Proteomics analysis and mechanism verification assays demonstrated that pretreatment with canola CFP produced effects primarily through maintenance of normal redox, mitochondrial function and lipid metabolism in cells. Our results indicate that canola CFP has great potential for preventing TBHP-induced vascular endothelial cell dysfunction.