Abstract
BackgroundInfectious bronchitis virus (IBV) is first to be discovered coronavirus which is probably endemic in all regions with intensive impact on poultry production. In this study, we used two-dimensional gel electrophoresis (2-DE) and two-dimensional fluorescence difference gel electrophoresis (2-DIGE), coupled with matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI-TOF/TOF-MS), to explore the global proteome profiles of trachea and kidney tissues from chicken at different stages infected in vivo with the highly virulent ck/CH/LDL/97I P5 strain of infectious bronchitis virus (IBV) and the embryo-passaged, attenuated ck/CH/LDL/97I P115 strain.ResultsFifty-eight differentially expressed proteins were identified. Results demonstrated that some proteins which had functions in cytoskeleton organization, anti-oxidative stress, and stress response, showed different change patterns in abundance from chicken infected with the highly virulent ck/CH/LDL/97I P5 strain and those given the embryo-passaged, attenuated P115 stain. In addition, the dynamic transcriptional alterations of 12 selected proteins were analyzed by the real-time RT-PCR, and western blot analysis confirmed the change in abundance of heat shock proteins (HSP) beta-1, annexin A2, and annexin A5.ConclusionsThe proteomic alterations described here may suggest that these changes to protein expression correlate with IBV virus' virulence in chicken, hence provides valuable insights into the interactions of IBV with its host and may also assist with investigations of the pathogenesis of IBV and other coronavirus infections.
Highlights
Infectious bronchitis virus (IBV) is first to be discovered coronavirus which is probably endemic in all regions with intensive impact on poultry production
IBV antibody detection and observed clinical signs All chickens exhibited respiratory clinical signs at about 4-14 dpi with the IBV ck/CH/LDL/97I P5 strain
For chickens in the control group and those inoculated with the IBV ck/CH/LDL/ 97I P115 strain, no respiratory clinical signs and no gross lesions were observed during the experimental period
Summary
Infectious bronchitis virus (IBV) is first to be discovered coronavirus which is probably endemic in all regions with intensive impact on poultry production. Coronavirus infection may cause inflammation, alter the immune and stress responses, and modify the coagulation pathways [3] Such profound functional and morphological changes in host cells are associated with significant changes in the patterns of expression of host cell genes. It has been used to study enveloped RNA viruses such as influenza virus, respiratory syncytial virus (RSV), parainfluenza virus (PIV), human metapneumovirus (hMPV), SARS-CoV, and mouse hepatitis virus (MHV) [11,12,13,14,15,16,17,18] It provides invaluable information on the cellular signaling pathways involved in either the cellular response to viral infections, or the viral manipulation of cellular machinery to ensure their own survival. The in vivo infection model could yield more biologically relevant insights into pathogenesis
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