Abstract
The natural polysaccharides extracted from the pollen of Pinus massoniana (TPPPS) have been shown to be a promising immune adjuvant against several viral chicken diseases. However, the exact mechanism through which TPPPS enhances the host immune response in chicken remains poorly understood. In the current study, chicken peripheral blood lymphocytes were treated with varying concentrations of TPPPS and pro-inflammatory cytokines such as IFN-γ, iIL-2 and IL-6 were measured to determine the optimal dose of the polysaccharide. A comparative analysis was subsequently performed between the proteome of lymphocytes subjected to the best treatment conditions and that of untreated cells. Protein identification and quantitation revealed a panel of three up-regulated and seven down-regulated candidates in TPPPS-treated chicken peripheral blood lymphocytes. Further annotation and functional analysis suggested that a number of those protein candidates were involved in the regulation of host innate immune response, inflammation and other immune-related pathways. We believe that our results could serve as a stepping stone for further research on the immune-enhancing properties of TPPPS and other polysaccharide-based immune adjuvants.
Highlights
Polysaccharides are structurally complex macromolecules with a wide range of biological functions, including the mediation of signal transduction, antibody-antigen recognition, bacterial invasion, etc. [1]
Treatment with TPPPS could enhance the immune activity of chicken peripheral blood lymphocytes
We subsequently compared the proteome of chicken peripheral blood lymphocytes treated with 100 μg/mL of TPPPS to that of untreated cells using the iTRAQ technique
Summary
Polysaccharides are structurally complex macromolecules with a wide range of biological functions, including the mediation of signal transduction, antibody-antigen recognition, bacterial invasion, etc. [1]. Polysaccharides are structurally complex macromolecules with a wide range of biological functions, including the mediation of signal transduction, antibody-antigen recognition, bacterial invasion, etc. Plant- and fungus-derived polysaccharides have attracted significant attention due to their low toxicity, excellent biocompatibility and potential therapeutic values [2]. These carbohydrate macromolecules have been used to construct tissue engineering scaffolds, which demonstrated controllable degradability and excellent. Proteomics analysis of chicken peripheral blood lymphocyte in TPPPS regulation analysis, decision to publish, or preparation of the manuscript
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