Proteomics Advances in the Understanding of Pollen-Pistil Interactions.

Ziyang Fu,Pingfang Yang

doi:10.3390/proteomes2040468

Ziyang Fu, Pingfang Yang

Open Access

https://doi.org/10.3390/proteomes2040468

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Journal: Proteomes	Publication Date: Sep 29, 2014
Citations: 83	License type: CC BY 4.0

Affiliation: Wuhan Botanical Garden, Chinese Academy of Sciences

Abstract

The first key point to the successful pollination and fertilization in plants is the pollen-pistil interaction, referring to the cellular and molecular levels, which mainly involve the haploid pollen and the diploid pistil. The process is defined as “siphonogamy”, which starts from the capture of pollen by the epidermis of stigma and ends up with the fusion of sperm with egg. So far, the studies of the pollen-pistil interaction have been explicated around the self-compatibility and self-incompatibility (SI) process in different species from the molecular genetics and biochemistry to cellular and signal levels, especially the mechanism of SI system. Among them, numerous proteomics studies based on the advanced technologies from gel-system to gel-free system were conducted, focusing on the interaction, in order to uncover the mechanism of the process. The current review mainly focuses on the recent developments in proteomics of pollen-pistil interaction from two aspects: self-incompatible and compatible pollination. It might provide a comprehensive insight on the proteins that were involved in the regulation of pollen-pistil interaction.

Highlights

Deep in the evolutionary history, the plant kingdom goes through trends from low to high, simple to complex, aquatic to terrestrial
The studies of the pollen-pistil interaction have been explicated around the self-compatibility and self-incompatibility process in different species from the molecular genetics and biochemistry to cellular and signal level, especially the generation mechanism of SI system [3,4,5]
arabinogalactan proteins (AGPs) were localized predominantly in the cell wall of secretory cell of the stigma, as well as in the transmitting tissue of the pistil during the pollination period by means of immunofluorescence localization. These results proved that proteins play roles of supporting pollen performance and tube growth during the pollination stage in olive, which corresponds to the conclusion that AGPs had roles in vegetative, reproductive, and cellular growth and development, previously [50,51]

Summary

Introduction

Deep in the evolutionary history, the plant kingdom goes through trends from low to high, simple to complex, aquatic to terrestrial. The studies of the pollen-pistil interaction have been explicated around the self-compatibility and self-incompatibility process in different species from the molecular genetics and biochemistry to cellular and signal level, especially the generation mechanism of SI system [3,4,5]. Proteomics, as the study of how proteins work, interact, diversify and specialize on a global scale, has been widely applied in analyzing the biological processes drawing support from the rapid development of mass spectrometry (MS). By these new and powerful proteomic techniques, the interaction between pollen and pistil can be improved and studied with new insight [7]. This review focuses on the advance of proteomics studies in the interaction between pollen and pistil to reveal the complex biological process from a pivotal gene or enzyme to an integral pathway of regulatory or signal transduction

Proteomic Analysis of Pollen-Pistil Interaction with Successful Fertilization

Protein Analysis on Pistils by Comparative Proteomics

Proteomic Analysis of Pollen-Pistil Interaction in SI Response

Proteomic Analysis of the Gametophytic Self-Incompatibility Response

Proteomic Analysis of the Sporophytic Self-Incompatibility Response

Findings

Conclusions and Perspectives