Proteomic Study Identifies Glycolytic and Inflammation Pathways Involved in Recurrent Otitis Media.

Blendi Ura,Domenico Leonardo Grasso,Fulvio Celsi,Luisa Zupin,Bartolomea Gaita,Ilaria Battisti,Raffaella Sagredini,Giorgio Arrigoni,Egidio Barbi,Sergio Crovella,Eva Orzan

doi:10.3390/ijms21239291

Abstract

Recurrent acute otitis media (RAOM) in children is clinically defined as the occurrence of at least three episodes of acute otitis media over a course of 6 months. A further common pathological condition of interest in the context of pediatric otolaryngology is adenotonsillar hypertrophy (ATH), a common cause of obstructive sleep apnea syndrome. Aimed at unraveling the differential modulation of proteins in the two pathologies and at understanding the possible pathways involved in their onset, we analyzed the proteomic profile of the adenoids from 14 RAOM and ATH patients by using two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS). The 2-DE coupled with MS allowed us to identify 23 spots with significant (p-value < 0.05) changes in protein amount, recognizing proteins involved in neutrophil degranulation and glycolysis pathways.

Highlights

Recurrent acute otitis media (RAOM) is a disorder in which a child experiences at least three episodes of acute otitis media over a course of 6 months or four episodes across 12 months [1]
Aimed at assessing the different proteins modified in RAOM and adenotonsillar hypertrophy (ATH), in the attempt to consider the molecular pathways involved in both diseases, we focused on a proteomic analysis in the adenoidal tissue, after surgical removal
We used 2‐DE and mass spectrometry (MS) analysis to compare the proteomic profiles of adenoidal tisIsnuethfirsomstuRdAy,OwMe uanseddA2T-DHEpaantidenMtsS

Summary

Introduction

Recurrent acute otitis media (RAOM) is a disorder in which a child experiences at least three episodes of acute otitis media over a course of 6 months or four episodes across 12 months [1]. The percentage of RAOM occurrence in children under 7 years of age has been estimated to be between 20% and 30% [3]; the quality of life of both children and parents is significantly affected, especially for the social limitations due to the disease. The clinical treatment of RAOM is complex and different strategies could be employed to reduce the frequency of the episodes, including mainly oral antibiotic treatment [1] and adenotonsillectomy. Both have limited efficacy on the recurrence of episodes [4]

Objectives

Methods

Results