Proteomic Responses of Wheat Roots during Enhanced Ultraviolet-B Radiation Stress

Abstract

Enhanced ultraviolet-B (UV-B) radiation is one of the most important abiotic stresses that could influence the growth and physiological traits of plants. Wheat (Triticum aestivum) is also affected by present-day enhanced UV-B radiation. As protein metabolism plays an important role in plant adaptation to UV-B radiation, this study was designed to identify UV-B -responsive proteins in wheat roots. Wheat (Lin Fen NO.3) was exposed to UV-B radiation with normal light (group B) and only normal light (group CK) in growth chambers. Roots were harvested at 3d, 6d and 9d after UV-B radiation. Proteins were extracted and separated by two-dimensional gel electrophoresis (2-DE). 15 protein spots were regulated by UV-B radiation at 6d. Among these protein spots, 3 proteins were up-regulated and 10 proteins were down-regulated. 2 proteins were expressed only in CK group. 3 protein spots were identified using mass spectrometry. They were further verified as Ribulose-bisphosphate carboxylase activase; Catalase (CAT); Hypothetical protein. These data support the assumption that UV-B may have a regulatory role besides damaging effects and that an increased UV-B environment will likely increase this regulatory influence of UV-B radiation.